Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells - Institut Gustave Roussy
Article Dans Une Revue Scientific Reports Année : 2016

Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells

Résumé

Nanofibers of sepiolite, a natural silicate belonging to the clay minerals family, might constitute a potential promising nanocarrier for the non-viral transfer of bio-molecules. We show here that sepiolite nanofibers efficiently bind different types of DNA molecules through electrostatic interactions, hydrogen bonding, cation bridges, and van der Waals forces. Moreover, Fourier-transform infrared spectroscopy identified the external silanol groups as the main sites of interaction with the DNA. Furthermore, as a proof of concept, we show that sepiolite is able to stably transfer plasmid DNA into mammalian cells and that the efficiency can be optimized. Indeed, sonication of sepiolite 100-fold stimulated DNA transfection efficiency. These results open the way to the use of sepiolite-based biohybrids as a novel class of nanoplatform for gene transfer with potential clinical applications.
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hal-03065105 , version 1 (17-04-2024)

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Fidel Antonio Castro-Smirnov, Olivier Pietrement, Pilar Aranda, Jean-Rémi Bertrand, Jeanne Ayache, et al.. Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells. Scientific Reports, 2016, 6, pp.36341. ⟨10.1038/srep36341⟩. ⟨hal-03065105⟩
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