Optimization of normal phase chromatographic conditions for lipid analysis and comparison of associated detection techniques - Université Paris-Saclay Accéder directement au contenu
Article Dans Une Revue Journal of Chromatography A Année : 2017

Optimization of normal phase chromatographic conditions for lipid analysis and comparison of associated detection techniques

Résumé

One important challenge in lipid class analysis is to develop a method suitable or, at least adaptable, for a vast diversity of samples. In the current study, an improved normal-phase liquid chromatography (NPLC) method allowed analyzing the lipid classes present in mammalian, vegetable as well as microorganism (yeast and bacteria) lipid samples. The method effectively separated 30 lipid classes or subclasses with a special focus on medium polarity lipids. The separation was carried out with bare silica stationary phase and was coupled to evaporative light scattering detection (ELSD), charged aerosol detection (CoronaCAD® ) and mass spectrometry. Solutions are provided to circumvent technical issues (such as pumping solvents of low viscosity, solvent purity, rinsing step). The influence of mobile phase composition and addition of ionic modifiers on the chromatographic behavior of particular lipid classes is documented. A comparison between ELSD and Corona-CAD® confirmed the interest of this later detector for samples with a wide range of concentration of different lipids. Three common atmospheric pressure ionization interfaces were used for coupling the NPLC separation to a LTQ Velos Pro® mass spectrometer. The comparison of the chromatographic profiles showed that atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) are both suitable to detect the different lipid classes whereas APPI allows a better sensitivity for lipids at low-concentration.
Fichier principal
Vignette du fichier
PostPrint JCA S ABREU 07 july 2017.pdf (1.86 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04529765 , version 1 (05-04-2024)

Identifiants

Citer

Sonia Abreu, Audrey Solgadi, Pierre Chaminade. Optimization of normal phase chromatographic conditions for lipid analysis and comparison of associated detection techniques. Journal of Chromatography A, 2017, 1514, pp.54-71. ⟨10.1016/j.chroma.2017.07.063⟩. ⟨hal-04529765⟩
8 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More