A synthetic communication system uncovers extracellular immunity that self-limits bacteriophage transmission - Laboratoire Interdisciplinaire des Sciences du Numérique Access content directly
Preprints, Working Papers, ... Year : 2022

A synthetic communication system uncovers extracellular immunity that self-limits bacteriophage transmission

Abstract

Understanding how delivery and exchange of genetic information by bacteriophages shapes bacterial populations is important for designing applications for phage therapy, biocontrol, and microbiome engineering. Here, we present a synthetic intercellular communication system that repurposes phage M13 for genetic exchange between Escherichia coli cells and build mathematical models of the communication behaviour. Our models, based on Chemical Reaction Networks, capture the growth burden, cell density, and growth phase dependence of phage secretion and infection kinetics and predict the stochasticity characterising phage-bacterial interactions at low numbers. In co-cultures of phage sender and receiver cells, resource sharing and selection pressure determine the choice of horizontal versus vertical phage transmission. Surprisingly, we discover that a phage-encoded immunity factor confers extracellular protection to uninfected bacteria, reducing infection rates by 70%. In a simulated gut environment, this novel “self-jamming” mechanism enables the phage to farm uninfected bacteria for future infections, increasing the overall success of both M13 and E. coli. The synthetic system developed here lays the groundwork for implementing population level controls in engineered bacterial communities, using phage signals for communication.

Domains

Biotechnology
Fichier principal
Vignette du fichier
2022-07-22 11h39 PhageCommModel MS v2 (M13 focus) - clean copy.pdf (1.58 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03670575 , version 1 (17-05-2022)
hal-03670575 , version 2 (10-08-2022)

Identifiers

Cite

Amit Pathania, Corbin Hopper, Amir Pandi, Matthias Függer, Thomas Nowak, et al.. A synthetic communication system uncovers extracellular immunity that self-limits bacteriophage transmission. 2022. ⟨hal-03670575v2⟩
255 View
65 Download

Altmetric

Share

Gmail Facebook X LinkedIn More