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Article Dans Une Revue Journal of Chromatography A Année : 2016

Alternative solvents for improving the greenness of normal phase liquid chromatography of lipid classes

Résumé

An evaluation of solvents alternative to n-heptane (d-limonene and hexamethyldisiloxane) and chloroform (cyclopentyl methyl ether, 2-methyltetrahydrofuran and isopentyl acetate) was developed for lipid classes separation of non-polar cholesteryl ester to highly polar phospholipids by high-performance liquid chromatography on bare silica stationary phase and evaporative light-scattering detection. Screening of alternative solvents was used to estimate their compatibility with liquid chromatography and evaporative light-scattering detection and to evaluate their chromatographic selectivity. This work shows that n-heptane can be advantageously replaced by hexamethyldisiloxane. An increase of non-polar lipids retention is observed with hexamethyldisiloxane as weak solvent. Chloroform, which is largely used for lipid analysis, might be replaced efficaciously by cyclopentyl methyl ether, 2-methyltetrahydrofuran or isopentyl acetate. Aside from offering a different selectivity, the gradients composed by one or both alternative solvents gave efficient and comparable or even better separations than those obtained with conventional solvents.
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Dates et versions

hal-04529781 , version 1 (02-04-2024)

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Nolwenn Prache, Sonia Abreu, Patrick Sassiat, Didier Thiébaut, Pierre Chaminade. Alternative solvents for improving the greenness of normal phase liquid chromatography of lipid classes. Journal of Chromatography A, 2016, 1464, pp.55-63. ⟨10.1016/j.chroma.2016.07.083⟩. ⟨hal-04529781⟩
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